
Sponge cells are transferred to cryogenic tubes, which are put into freezer containers containing isopropyl alcohol that slowly freeze the cells at a rate of 1°C (34°F) per minute until they reach -80°C (-112°F).
Sponge cells are transferred to cryogenic tubes, which are put into freezer containers containing isopropyl alcohol that slowly freeze the cells at a rate of 1°C (34°F) per minute until they reach -80°C (-112°F).